Some people with more severe allergies to wasp stings may go into anaphylactic shock, which can be life threatening. You should let your doctor know right away if you have a more concerning reaction after a wasp sting. Most times, these reactions will subside on their own in about a week. Symptoms to note: More concerning reactions to wasp stings, that may indicate some level of allergy to the venom, include extreme redness and swelling that increases for two or three days after the sting. Usually, the pain and swelling recedes within several hours of being stung. A tiny white mark may be visible in the middle of the welt where the stinger punctured your skin. Often, a raised welt will appear around the sting site. Typical wasp stings present as sharp pain, redness, swelling, and itching or burning at the sting site. What it looks like: Wasp stings can be uncomfortable, but most people, without wasp sting allergies, recover quickly and without complications. Ready to get to the bottom of your bug bite mystery? Here are pictures of the most common bug bites to keep on your radar. ( Spiders, bed bugs, and fleas don’t hibernate in the winter, sadly.) There are ways to deter the critters from coming your way, especially if you use insect repellent or bug spray, but there’s no perfect solution to ward off the pests.Īlthough it’s not always possible to know which bug is to blame for your latest bite (or bites), you can often get a pretty good idea based on your skin, the site of the sting, and a few other clues. You may notice that bug bites pick up in the summer, when you’re outside more often and exposing more skin, but they can occur any time of year-especially if you happen to be preyed upon inside of your home. It’s possible you could be having a severe allergic reaction, suffering from an insect-borne disease, or experiencing an infection. Mild itchiness and pain are normal reactions to insect bites and stings, but if you experience anything more severe than these, including shortness of breath and swelling away from the original site, you should contact a doctor or seek medical attention immediately. Everyone’s immune system reacts to bug bites and stings differently, producing unique symptoms in each victim, from swelling to itching to pain. In fact, experts say that it’s hard to positively identify the offending insect unless you actually catch it in the act. Unfortunately, experience shows us that almost all bug bites look strikingly similar. Have a nasty bite you can’t attribute to a specific house bug or outdoor pest? We’re sharing bug bite pictures to help you properly identify the offender, and how to best treat the wound. And often, you haven’t the slightest clue which critter could have been the culprit. Some ache, some beg to be scratched, and the worst of the bunch do both. Whether you first notice a sharp pinch, a nagging itch, or a painful sting, discovering a brand-new bug bite-sometimes instantly, but often only days later-is no fun.
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But this section is where you’ll notice some big differences. You fill out the month as you go and use it to write in big-picture goals or upcoming events. Monthly sectionīoth the Best Self Journal and the Panda Planner have an undated monthly section at the front of the book. There, you’ll get walk-throughs from Panda Planner’s founder and CEO. It’s a step-by-step to-do list of sorts that you can refer back to when filling out the planner.įor more in-depth tutorials, you’ll want to watch the quick start videos, which include videos on the daily, weekly, and monthly sections of the planner. The Panda Planner, on the other hand, keeps it simple with one page that details how to use the planner to be more productive and, ultimately, happier. It’s where the Journal asks you to fully commit your life to these goals over the next 13-weeks by reminding you how your life will improve by achieving these particular goals. For example, you might have a sales goal, a marketing goal, and a fitness goal. There’s space for three separate goals, so you can outline three important areas of your life you want to improve. There’s a quick-start guide at the beginning, but it’s best to read through the entire thing, to get the full gist of it.Īlso, at the front of the Best Self Journal is a four-page, 13-week roadmap that helps you understand the meaning behind your big goal and break it into actionable steps. The Best Self Journal comes with a booklet that details how to use the planner to its fullest. However, there are some stark differences. Both focus on research, incorporate strategies based on science like gratitude and habit tracking, and encourage regular reflection to help you accomplish your goals. That’s not where the similarities end though. Design and layoutīoth the Panda Planner Classic and the Best Self Journal are 13-week planners with undated Daily, Weekly, and Monthly sections for you to plan your time. Panda Planner also comes with a ton of online resources via, which include eBooks, Quick Start Guide videos, and a robust online Facebook community to keep you motivated. Other than that, the packaging is pretty streamlined. There are instructions for using the planner to the fullest on the first page, as well as the science behind the layouts and processes. The Panda Planner, however, is much simpler. You also get access to their online community, which touts hundreds of thousands of users cheering you on to reach your goals. With the Best Self Journal, you get a 13-week dry-erase wall calendar, a book telling you how to use the journal, and a card to plan your daily morning and evening routines. The box is beautiful and comes with a few extras the Panda Planner doesn’t. While the Best Self Journal is a little more expensive, it comes across in the packaging. So the Panda Planner is about $10 cheaper after all is said and done. So if you’re a Prime member you can score a deal with free two-day shipping. The Best Self Journal also charges a shipping fee, while Panda Planner is available on Amazon Prime. At only $24.97, the Panda Planner Classic comes in a few bucks cheaper than the Best Self Journal’s $31.99. If price is a huge factor for you, both these planners are great options. However, there are some major differences, which is why we’re going in-depth in our Panda Planner and Best Self Co Journal review - so you can choose wisely. What Works Best For You? Best Self Journal or Panda Planner® Classicīoth planners are daily productivity tools backed by science and touted by entrepreneurs for their effectiveness. Today, we’re breaking down two of the best productivity planners on the market: Best Self Journal by Best Self Co and Panda Planner® Classic. And sure, there are a lot of options out there. One way to push the pedal on all of these aspects of productivity? Finding, and then using, the right tools. But how do we get there? Through prioritization, organization, strategic scheduling, time and energy management, finding meaning in our work, and the list goes on. Let’s face it - we all want to be more productive. This means that you can change the language and font of the program, select different safety measures, change animation and file format colors, and edit the layout of the program. The top band also includes the settings using which you can personalize the program. Detailed information on all scans appeared on the bottom of the central panel. You can use the options available on the top to open any particular file or folder that you wish to scan. The window that appears on launch consists of a central panel that shows folder statistics and the left panel shows a list of available drives. SpaceMonger has an intuitive interface that’s easy to maneuver. You can change the way you see everything, and also open and delete different files directly from the interface. It helps you understand what’s occupying space, so you can effectively get rid of large files without worrying about deleting something important. Once downloaded, the complex software scans your device and creates a treemap of all folders, sub-folders, and files. It can show you a graphic representation of what’s taking up the most space. Not only is the process a bit time-consuming, but it can also have you deleting files that won’t make a significant difference. You have to manually go through the properties of all folders and sub-folders, open files to check whether or not they’re important, and delete whatever isn’t required. What is SpaceMonger?įreeing up hard drives can be quite tedious. The program comes with a free trial period that doesn’t require any registration. Designed for Windows, SpaceMonger download features treemaps, statistics, and pie charts, as well as an option to connect with cloud storage. With multiple viewing options, the application lets you easily find files and folders taking up the maximum space on your hard disk. SpaceMonger is a disk analysis utility tool that helps users get a clear picture of their computer’s storage space. Cathy Buggs Updated 4 months ago Find files taking up disk space Amazon is offering millions of products at everyday low prices across categories including Books, Video Games, Consumer Electronics, Baby and Toys, and Home and Kitchen, both from iconic international brands like Bose, Fisher Price, Kitchenaid, LEGO, L'Oreal, and Spigen, and from Singapore businesses including Biofinest, Creative, KeaBabies and Skin Inc. Singapore customers can now shop anywhere, anytime through desktop and mobile browsers as well as the Amazon Shopping app. More ways to shop millions of products with the convenience Amazon customers have come to love "Today marks the next step for us in Singapore, and we will continue to work hard to earn the trust of Singapore customers by growing our product range, ensuring low prices, and providing a convenient and trusted shopping experience." And we are also adding even more benefits for Prime members", says Henry Low, Amazon Singapore Country Manager. "With Amazon.sg, we want to provide what customers have been asking us for: the ability for everyone to shop on desktop and mobile, more local and international selection from Amazon and trusted sellers, paired with fast and reliable delivery. Customers who sign up for Prime receive free one-day delivery on local selection with no minimum spend, and free international shipping on eligible orders over S$60 for Amazon International Store products. All customers in Singapore enjoy free delivery on eligible orders over S$40 within 2-3 days. Customers can buy millions of products from categories such as Books, Video Games, Consumer Electronics, Baby and Toys, and Home and Kitchen. 8, 2019 /PRNewswire/ - Amazon today announced the launch of Amazon.sg, introducing more ways to shop with Amazon in Singapore - on desktop, mobile browser and shopping app. On Amazon.sg, small and medium sized businesses can offer their products to online shoppers across Singapore There, you must spend Incredibricks - a collectable found throughout the city, which you can find easily by clearing out Crime Waves - to unlock them. To unlock them, you need to find specific Pixar Family Build locations across the town. There are many characters to unlock across LEGO Incredibles, but the most interesting are those from other Pixar films. LEGO Incredibles Pixar character locations list Completing Challenges or Crime Waves or Pixar Family Builds will give you free Mystery Bags that you open right away. In this game, you get them for doing various things, and they give you new characters. Those have to be unlocked in-game, as discussed in the subsequent section below. In real life, LEGO Mystery Bags are opaque bags that give you a mystery minifigure based on a LEGO property. LEGO The Incredibles Achievements Best Vacation Ever achievement LEGO The Incredibles 1,552 1,000 51 4.01 21,078 3,731 (18) 15-20h Best Vacation Ever achievement in LEGO The. If successful, you'll see a notification, like so: LEGO Incredibles cheat codes listĪs with LEGO Star Wars Force Awakens cheat codes, the codes available in LEGO Incredibles only unlock additional characters - just two in total - but don't give you bonus bricks or comical powers. As there are many logical elements in the game, the guide contains a full walkthrough of all of the 12 levels of the main storyline. To enter the below codes, pause the game at any time - even during the start of the first level - and select Extras then Enter Code from the pause menu.įrom there, you can enter the codes listed below. The guide to Lego The Incredibles describes the next game from the LEGO franchise, a highly popular one among both younger and older players. Where to enter LEGO Incredibles cheat codes If you're a fan of LEGO games in general, then we have other cheats pages, including LEGO Star Wars Force Awakens cheat codes, LEGO Harry Potter cheat codes, LEGO Marvel Super Heroes cheat codes and LEGO Incredibles cheat codes pages. There are, of course, many other things to unlock - including characters from other Pixar films - but you'll have to put some time and effort into it first. From there they will call you from the number you entered during this process. Usually, these codes unlock a range of characters and cheats, but LEGO Incredibles codes are a little different, or more accurately, scant - with just two codes in the game to enter. Hit the Touch Pad, hit R1 twice, and you'll be at the Challenges screen. You can find reference to these challenges on the Map Screen. LEGO Incredibles cheats allow you to unlock a few things without the effort of playing the game - and since LEGO games are massive timesinks, this is no bad thing. In addition to the collectibles and all the mucking about the city, this game saw fit to give you a few extra challenges to take care of while in the city hub. Jay-Z has four bottles of Belvedere vodka and marijuana in abundance Jay-Z has six attractive women and six bottles of Cristal champagne Jay-Z quotes a line from the song 'Bittersweet Symphony' to suggest that the woman is a mistakeĭespite the negative feelings expressed in the previous line, Jay-Z still orders more drinks Jay-Z and the woman are both under the influence and singing badly Jay-Z is boastfully indicating that a model cannot resist him Jay-Z is inebriated from drinking Cristal champagne and is around a woman who is also high Jay-Z is confident that he can easily replace any woman Jay-Z sees himself as a pimp by nature, not by family ties People are speculating about whether Jay-Z will choose to have sex with or reject someone Jay-Z suggests he might not be himself when Champagne is involved Jay-Z is warning the woman not to lie or pretend Jay-Z wants a woman to give him her all, both physically and emotionally Jay-Z believes that his wealth and success will make a woman forget about her current partner Jay-Z recognizes that having money can come with its own set of issues Jay-Z wants to be loved for who he truly is Jay-Z is expressing his desire to love someone Jay-Z is looking towards the future, striving for more success Jay-Z is stating that his past doesn't define him Jay-Z is letting his listeners know that he wants them to understand something important I just want you to know (Wanna let you know) Jay-Z is declaring that he is a hustler, he works hard for his success He is untouchable and part of a powerful dynasty Jay-Z is cementing his status in the hip-hop industry through these titles and nicknames. Jay-Z is warning his listeners that he's about to come with a strong performance Repetition of Jay-Z's nickname, showing his confidence Jay-Z is hyping himself up to start the songĪ shortened version of Jay-Z's nickname, 'Jigga-Hov' Overall, "I Just Wanna Love U" showcases Jay-Z's confidence, desire for love, and power in the music industry. However, as the song progresses, he reveals his softer side, talking about his desire for true love and emotional support. The lyrics "When the Remi's in the system, ain't no tellin'/Will I fuck 'em will I diss 'em, that's what they be yellin'" reflect on Jay-Z's social power and reputation as a womanizer who can get whatever he wants. The song also tackles themes of success and power, emphasizing Jay-Z's position in the music industry as an unstoppable force. The song also talks about the price of fame, as well as Jay-Z's tendency for womanizing and materialism. The song's chorus "Gimme that funk, that sweet, that nasty, that gushi stuff" reflects his desire to have a relationship that is passionate, honest, and fulfilling. The lyrics highlight Jay-Z's confidence and his capability to get whatever he desires. The song reflects on the rapper's status as a successful hustler and how all the money he has earned has not changed his desire for love. Jay-Z's "I Just Wanna Love U" is an upbeat song about love and money. Same song, I'm back, been around the world I'm too cold, Motorola, two way page me, c'mon Ladies love me long time like 2Pac's soul Profess you love the Hov', and I'll never let you down Know how to work your hips and your head's priceless Might light your wrist, but that about itįuck it, I might wife you and buy you nice whips How, much I'ma spend but you already know Let's keep it real ma, you savin' it for karats Yeah, save the narrative, you savin' it for marriage Mama, gimme that funk, that sweet, that nasty, that gushi stuff Go somewhere private where we can discuss fashion What do you say, me, you, and your Chloe glasses Got six model chicks, six bottles of Crist' Will I fuck 'em will I diss 'em, that's what they be yellin' When the Remi's in the system, ain't no tellin' Gimme that funk, that sweet, that nasty, that gushi stuffĬ'mon, gimme that funk, that sweet, that nasty, that gushi stuff Now I just wanna love you (just wanna love you)Īnd with all this cash (mo' money, mo' problems) It ain't where I been (It aint where I been)īut where I'm bout to go (Top of the world!) Version 6.1.2 is still available for download on their FTP site, as are versions 4.6, 4.1, and even version 3.5, for you holdouts still clinging to System 6. As of February 25, 2003, BBEdit Lite has been discontinued and is no longer available…. Thank you for your interest in BBEdit Lite. Anyone currently using the software is welcome to continue doing so, in accordance with the existing license agreement…. This chart is not an exhaustive listing of BBEdit’s features. All of the highlighted features are available during the initial 30-day evaluation period. BBEdit Lite has been discontinued and is no longer being developed or supported. This chart shows what you get when you buy a BBEdit license, compared to the features available when you use BBEdit without a license after the 30-day evaluation period. Just to prove I wasn’t smoking crack when I wrote the above eulogy for BBEdit Lite, a comparison between yesterday’s update to the BBEdit Lite web page and the version in my local cache from a few days ago shows the difference: BBEdit is ranked 8th while TextWrangler is ranked 11th. When comparing MacDown vs BBEdit, the Slant community recommends. In the question What are the best programming text editors for a Mac with a GUI. Also known as TextWrangler, BBEdit is a simple and lightweight code editor for macOS. Their own side-by-side comparison with the fully functional demo ofĪlso new is a comparison page showing what is different between When comparing BBEdit vs TextWrangler, the Slant community recommends BBEdit for most people. Anyone currently using the software is welcome to continueĭoing so, in accordance with the existing license agreement.Īdditionally, anyone may obtain a copy of BBEdit Lite to conduct Bottom Line: Which is Better - TextWrangler Text Editor or BBEdit BBEdit is more expensive to implement (TCO) than TextWrangler Text Editor, and BBEdit is rated with the same score (62/100) as TextWrangler Text Editor (62/100). Its existing license agreement, through several software update Compare TextWrangler Text Editor and BBEdit - Jun 2022 Compare Pricing. According to a Bare Bones press release yesterday (not yet on their web site):Īlthough BBEdit Lite is no longer developed or supported by Bareīones Software, it remains readily obtainable, under the terms of There is not a free version of BBEdit available.īare Bones has changed their policy (or at least clarified it): while BBEdit Lite is no longer being developed or supported, it is still available, legally, and apparently always will be. This program may be useful for writing a manuscript or an abstract with a strict limit on the number of words or characters allowed.Ends up this comment from my initial look at TextWrangler is no longer true:Īnd so today is the first day since BBEdit 2.1 shipped in April 1992 that Word Counter can handle various file types including plain text (txt, text), rich text (rtf, rtfd), Hypertext Markup Language (htm, html), Microsoft Word (doc), Microsoft Word XML (wordml), Apple's Web archive (webarchive), the Adobe Portable Document Format (pdf), and others. Word Counter can also calculate estimates for readability statistics using the well known Flesch-Kincaid readability formula and many others. so is best avoided TextWrangler: Not just a file comparison utility. It can even create a sortable summary table of all words in the document, with the number of times each word appears and the length of each word. If you already have a file comparison utility installed on your computer. Word Counter can count the number of times a particular word appears in a document. You can use a text editor for a wide variety of tasks from cleaning up data, to editing configuration files on your Mac or server, to writing HTML or coding. At its most basic, a text editor is a tool for simply editing text. It also can perform bulk counts on multiple files and folders simply by dropping them onto the window. TextWrangler is the powerful general purpose text editor, and Unix and server administrator’s tool. commit-archive commit-revisions community community-list compare-routerid. It can show the progress achieved towards a set goal for the total number of words and characters. For you Mac users with BBEdit or TextWrangler, here is a Language Code Module. Word Counter can automatically update the count based on a user-defined time interval. It can be used independently or in conjunction with other applications such as TextEdit, Microsoft Word, Pages, TextWrangler, and others. Word Counter is an application that performs a word count and a character count, but it can do much more. Turn of the Tide - NETFLIX SERIES - 5/26/23 The Ultimatum: Queer Love - NETFLIX SERIES - 5/24/23īarbecue Showdown: Season 2 - NETFLIX SERIES - 5/26/23 Wanda Sykes: I'm An Entertainer - NETFLIX COMEDY - 5/23/23 Victim/Suspect - NETFLIX DOCUMENTARY - 5/23/23 MerPeople - NETFLIX DOCUMENTARY - 5/23/23 The Creature Cases: Chapter 3 - NETFLIX FAMILY - 5/22/23 Comedy viewers have a surprise in store as well, as Wanda Sykes: I’m An Entertainer brings the acerbic comedian back for a streaming special poking fun at the post-pandemic world.Īlso, for reality fans who have been waiting for The Ultimatum: Queer Love to debut after its sneak peek on Love is Blind, the ongoing series kicks off its debut in this week’s crop of titles. He’s back again! Arnold Schwarzenegger returns to action yet again, as the series we saw teased in Netflix’s FUBAR trailer makes its way to the platform in this week’s May additions. (Image credit: Christos Kalohoridis/Netflix) New On Netflix The Week Of May 21: FUBAR, Wanda Sykes: I’m An Entertainer, The Ultimatum: Queer Love, And More Young, Famous & African: Season 2 - NETFLIX SERIES - 5/19/23 Selling Sunset: Season 6 - NETFLIX SERIES - 5/19/23 Kathal - A Jackfruit Mystery - NETFLIX FILM - 5/19/23 Yakitori: Soldiers of Misfortune - NETFLIX SERIES - 5/18/23Īstérix & Obélix: The Middle Kingdom - NETFLIX FILM - 5/19/23 Working: What We Do All Day - NETFLIX DOCUMENTARY - 5/17/23 Rhythm + Flow France: Season 2 - NETFLIX SERIES - 5/17/23 McGREGOR FOREVER - NETFLIX DOCUMENTARY - 5/17/23 One is a bittersweet journey, the other is a complicated examination, and both will teach the world a little more about those respective subjects.Īnna Nicole Smith: You Don't Know Me - NETFLIX DOCUMENTARY - 5/16/23įaithfully Yours - NETFLIX FILM - 5/17/23 Through Anna Nicole Smith: You Don’t Know Me and McGregor Forever, Netflix subscribers will be able to look deeper into the lives of these very different public figures. (Image credit: Netflix) New On Netflix The Week Of May 14: Anna Nicole Smith: You Don’t Know Me, McGregor Forever, And Moreĭocumentary fans have this week’s Netflix lineup to thank for scratching that particular itch. Queer Eye: Season 7 - NETFLIX SERIES - 5/12/23 Ultraman: Season 3 - NETFLIX ANIME - 5/11/23 Royalteen: Princess Margrethe - NETFLIX FILM - 5/11/23 Queen Cleopatra - NETFLIX DOCUMENTARY - 5/10/23 Missing: Dead or Alive?- NETFLIX DOCUMENTARY - 5/10/23 Hannah Gadsby: Something Special - NETFLIX COMEDY - 5/9/23ĭance Brothers - NETFLIX SERIES - 5/10/23 Spirit Rangers: Season 2 - NETFLIX FAMILY - 5/8/23 Justice League Unlimited: Seasons 1-2 - 5/8/23 However, if your mom is more of a person who likes to cry at heartwarming and cathartic developments, Hannah Gadsby: Something Special and Queer Eye Season 7 will both be up for the task. Abandoning her daughter in the name of her protection, a top notch assassin must reunite with her child in the ultimate showdown. Lo is about to go black ops in the Netflix original movie The Mother. (Image credit: Eric Milner/Netflix) New On Netflix The Week Of May 7: The Mother, Queer Eye: Season 7, Hannah Gadsby: Something Special, And More Queen Charlotte: A Bridgerton Story - NETFLIX SERIES - 5/4/23 Great British Baking Show: Juniors: Season 7 - NETFLIX SERIES - 5/3/23 Jewish Matchmaking - NETFLIX SERIES - 5/3/23 Lemony Snicket's A Series of Unfortunate Events - 5/1/23 The Curious Case of Benjamin Button - 5/1/23 The lighthearted vibes continue at the beginning of May, as the entire Austin Powers trilogy and Tom Hanks’ curmudgeonly comedy A Man Called Otto both take the stage in this first frame of May madness.Īustin Powers: International Man of Mystery - 5/1/23Īustin Powers: The Spy Who Shagged Me - 5/1/23 Jumping back in time to when the younger sovereign married her husband, King George III, another tale of betrothal and romance is about to unfold. “Surviving R.(Image credit: Niko Tavernise / CTMG, Inc.) New On Netflix The Week Of May 1: Queen Charlotte: A Bridgerton Story, A Man Called Otto, The Austin Powers Movies, And Moreīridgerton fans should be excited for Netflix’s May 2023 update, as Queen Charlotte: A Bridgerton Story finally debuts. “Thomas & Friends: The Mystery of Lookout Mountain” (Season 1) See the full list of titles coming to Netflix this month: Netflix is also slating several non-original films for April, including “Conan the Destroyer” and “The Last Stand” starring Arnold Schwarzenegger, along with the “Bourne Identity” trilogy, “Spider-Man” trilogy and an extended cut of Quentin Tarantino’s “The Hateful Eight.” To make it more fun and active, we had the catcher hula hooping, while waiting on the throw. This is probably best with the 2nd graders. The teacher will need to go around and help to make sure they are giving proper feedback. So you can tell them that today in class we are working on the underhand through and we are focusing on the follow through "fingers pointed to the target after ball leaves hand, waist height" so students can say that to their classmate. To make it even more meaningful have the catcher be the one who gives the feedback to their classmates. Throughout the activity make sure the teacher gives specific feedback about the the throwing cue you are working on like "Nice step with the opposite foot, Sally", "Way to follow through to the target, Robert". Once the catcher makes it to the other side of the floor, they will switch with the thrower. If unsuccessful, the thrower will try again. If successful, the catcher will scoot their poly spot back 2 steps. The thrower will perform the underhanded throw to try and get their ball into the hula hoop. The catcher will have the hula hoop around the waist. One student will be the thrower and the other will be the catcher. Spread the students out 15 feet from each other, and have them face each other. (If you do not have plicker magnets they can still do this activity and just switch out their type of equipment when they want.) Game continues until time is up or if one team has "no more" bean bags.A classic game turned into an activity. Students have 10-12 minutes to see how many different balls they can catch while using the plicker magnets to track their progress. Partners will then go and exchange their equipment for a new type ball and try and throw and catch 10 more and repeat the process of grabbing a bean bag from their teams basket and running it to the opposite teams basket. Once they throw and catch 10 balls with their partner (does not have to be in a row) they run to their teams basket and grab one bean bag (each) and run it to the other teams basket on the other side of the gym. When the music starts student's begin to overhand throw with their partner. instructions, warm-up, lesson, cool-down):ĭivide students in to two. Benefits/Explanation/Real-World Connection: Activities (i.e. You will need to explain how they will self assess with their partner with their plicker magnets. Must have previously taught overhand throw and catching skill cues. _X_ YES _NO Instructional Plan: Anticipatory Set/Pre-Activity: Green baskets will have different types of balls in them for students to choose from. Screen_Shot_at_2.16.18_PM.png Description of equipment set up:Ĭones are to divide the class in two teams.īlue baskets will have bean bags in them. This entire lesson is designed for 50 minute class. Plicker magnets Time Required for Lesson: (A) Teacher Planning: Equipment/Materials Needed: PE.5.1.3.d Catches a struck ball at all levels (e.g., overhand and underhand) in a non-dynamic environment. PE.5.1.3.b Throws overhand with accuracy in small-sided games. PE.4.1.3.e Catches a thrown ball at various levels (e.g., overhand and underhand) using a mature pattern in a non-dynamic environment. PE.4.1.3.c Throws to a partner or target with accuracy at a designated distance. PE.4.1.3.b Throws overhand using a mature pattern in a non-dynamic environment. PE.3.1.3.d Catches a tossed ball from a partner demonstrating 4 of 5 critical elements. PE.3.1.3.b Demonstrates 3 of 5 critical elements of an overhand throw in a non-dynamic environment for distance and/or force. Students will demonstrate correct skill cues for Students will be challenged to see how many different types of balls they can throw and catch 10 times with a Students can self assess their progress or teacher can assess skill cues during the activity No More. This is a lesson designed for overhand and catching skills. There are 4 disks containing sequence files. Each group should choose one of the sequenceįiles on the disk, and copy it from drive A to the desktop. Also copy the file pstblue1vector.txt toĬlick on Start, Programs, and Bioedit. Should recognise it as an ABI Autosequencer Trace file and open it as aĪdjust the size of the chromatogram trace with the Horizontal (You may have to scroll down the programĬlick on File menu, Open. You should be able to clearly see the peaksĬlick on the view menu, and check editable Scale and Vertical scale bars to the top left of the image. The computer will already have called most of the bases from Sequence that can be edited without changing the original sequence. Is colour-coded to match the colour that one of the 4 bases is displayed Should be able to make a visual judgement about which base should be present Still see some “N”s in the sequence where the computer cannot make a call. The sequence present in the original file is the sequence of Preferably, your own disk or network account.) That sequences after 400-500 bases become increasingly unreliable, and are notĬlick on the File menu, Export as text. Sequence complementary to the beginning of your original unedited forward Note that this is also displayed in a 5'-3' direction, so the The sequence of the original template strand, the Reverse Complement mustĬlick on the view menu (for the original uneditedįile), and check Reverse Complement. Save the reverse complement as a text file Sequence will be at the end of the reverse complement. (See sequence analysis references for fullĮither the T7 or SP6 promoter primers that flank the multiple cloning site inĪt the beginning of each sequence file you have are vector sequence, rather The sequences you are working with were prepared by theĭavidson lab from DNA fragments cloned in the pSTBlue-1 plasmid vector. To identify vector sequences, alignments will be preparedīetween your edited forward and reverse complement sequences and the sequence Interfere with analysis of the sequence, these will have to be edited out. (This file contains the sequence of the multiple cloning site region ofĬlick on the File menu, Import. Repeat this process for the pstblue1vector.txt Click on the edited forward sequence file to You saved the edited sequence files), files of type All files. Mouse by dragging it over the file names at the left. There should be a region of (almost) exact homology with the end of the reverse Vector sequence given is the opposite strand to the forward sequence, then This does not occur, repeat the process with the reverse complement sequence The vector should have a region of exact (or almost exact) homology with the If the vector sequence is on the same strand as the forward sequence, Click on Sequence menu, Pairwise alignment, Align two sequence (allow ends to slide). Manually edit and curate automatically generated alignments.Visualize alignments for figures and publication.Aid general understanding of large-scale DNA or protein alignments.Multiple alignment visualization tools typically serve four purposes: Identify the region of vector sequences.This page is a subsection of the list of sequence alignment software. The rest of this article is focused on only multiple global alignments of homologous proteins. The first two are a natural consequence of most representations of alignments and their annotation being human-unreadable and best portrayed in the familiar sequence row and alignment column format, of which examples are widespread in the literature. |
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